SAP30L (Sin3-associated Protein 30-like)

A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.\n\nThe transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kD and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22kD. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or product or post-translational modification.\n\nApplications:\nWestern Blot: Use at 1:500-1:1000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0095-10

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Lindfors, K. et al. BMC Genomics 4 (1), 53 (2003).

Additional Info

Reacts with residues 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L. Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein as well as endogenous hSAP30L in human cell lines (e.g., HEK293 and MCF-7 cells). Detects wild-type transfected hSAP30L or GST-hSAP30L fusion protein in non-human cell lines (e.g., NIH3T3 cells), but does not detect endogenous non-human SAP30L.