SAP97 (Disks Large Homolog 1, Synapse-associated Protein 97, SAP-97, DLG1, hDlg)

Synapse-associated protein (SAP) 97 is a 97kD membrane-associated synapse protein with extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal. SAP97 consists of three 90aa PDZ domains at its amino terminus, followed by a Src homology (SH) 3 domain, and a region with homology to guanylate kinases (GK). In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP97 is recruited to the submembranous cortical cytoskeleton at cell-cell contact sites as part of the E-cadherin complex. Co-expression of SAP97 and K+ channel results in the colocalization of these proteins in large round intracellular aggregates. SAP 97 has been shown to have functional homology to Drosophila tumor suppressor protein (DlgA), a PSD 95 related protein involved in maintaining integrity of epithelial and neuromuscular junctions.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:16,000\nWestern Blot: 0.3-1ug/ml, observed in lysates of cell line KELLY on ~150kD bands\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0095-27A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Mahoney ZX, Sammut B, Xavier RJ, Cunningham J, Go G, Brim KL, Stappenbeck TS, Miner JH, Swat W. Discs-large homolog 1 regulates smooth muscle orientation in the mouse ureter. Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19872-7. Epub 2006 Dec 15.

Additional Info

Recognizes human DLG1. Species sequence homology: Mouse and rat.