SAP97 (Synapse-associated Protein 97, Discs Large Homolog 1, DLG1, dJ1061C18.1.1, DKFZp761P0818, DLGH1, HDLG, Presynaptic Protein SAP97)

Synapse-associated proteins SAP97 and SAP102 display extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminals. In addition to the CNS, SAP97 is also detected at the basal lateral membrane between a variety of epithelial cells. SAP102 associates with NMDA receptors through the C-terminal NR2B subunit of the receptor. The N-terminal SAP97 PDZ domain mediates its interaction with the synaptic ras-GTPase activating protein SynGAP.\n \nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and \nElectron Microscopy. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1000 (ECL). Detects bands at ~97kD and 140kD.\nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nRat Brain Tissue Extract, \nMouse Brain Tissue Extract \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S0095-30

Size

25ug

Applications

ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS pH7.2, 0..09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Muller, B.M., Kistner, U., Veh, R.W., Cases-Langhoff, C., Becker, B., Gundelfinger, E.D., Garner, C.C. (1995) J. Neurosci. 15: 2354-2366. 2. Reuver, S., Garner, C. (1998) J. Cell Sci. 111: 1071-1080. 3. Kim, E., and Sheng, M. (1996) Neuropharmacolog 35: 993-1000. 4. Thomas, U., Phannavong, B., Muller, B., Garner, C.C., Gundelfinger, E.D. (1997) Mech \nDev. 62: 161-174. 5. Suzuki, T., Ito, J., Takagi, H., Saitoh, F., Nawa, H., Shimizu, H. (2001) Brain Res Mol Brain Res 89(1-2): 20-28. 6. Mehta, S., Wu, H., Garner, C.C., Marshall, J., (2001) J Biol Chem 276(19): 16092-16099. \n

Additional Info

Recognizes rat SAP97. Species Crossreactivity: human, mouse, canine.