SAPK, JNK, phosphorylated (Thr183 Tyr185) (Stress-Activated Protein Kinase, Jun-terminal Kinase)

The stress-activated protein kinase/Junterminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of an MAPKKK, typically MEKK1-4, or a member of the mixed lineage kinases (MLKs) that would phosphorylate and activate MKK4-7, the SAPK/JNK kinase. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4-7 can be activated in a pathway independent of small GTPases via stimulation of a member of the germinal center kinase (GCK) family. There are three SAPK/JNK genes with further diversification from alternative splicing. SAPK/JNK, when active as a dimer, can translocate to the nucleus where it regulates transcription through its effects on c-Jun, ATF-2 and other transcription factors.\n\nApplication:\nSuitable for use in ELISA, Western Blot, Immunoprecipitation, and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:2000. Incubate membrane with diluted S0096-04C in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:250\nFlow Cytometry: 1:200\nOptimal dilutions to be determined by the researcher.\n\nSource: Ascites\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0096-04C

Size

200ul

Applications

ELISA, FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1. Davis, R.J., Biochem. Soc. Symp. 64: 1-12 (1999). 2. Ichijo, H., Oncogene 18: 6087-6093 (1999). 3. Kyriakis, J.M., Avaruck, J., Physiol. Rev. 81: 807-869 (2001). 4. Kyriakis, J.M., J. Biol. Chem. 274: 5259-5262 (1999). 5. Leppa, S., Bohmann, D., Oncogene 18: 6158-6162 (1999). 6. Whitmarsh, A.J., Davis, R.J., Trends Biochem. Sci. 23: 481-485 (1998). 7. Akagi, T., et al., PNAS USA 97: 7290-7295 (2000). 8. Saporito, M.S., et al., J. Neurochem. 75: 1200-1208 (2000). 9. Somasundaram, S., et al., Cancer Res. 62: 3868-3875 (2002). 10. Song, J.S., et al., J. Immunol. 163: 802-810 (1999). 11. Xing, J., et al., Mol. Cell. Biol. 18: 1946-1955 (1998). 12. Zhang, S., Kaplan, M.H., J. Immunol. 165: 1374-1380 (2000).

Additional Info

Detects endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species Crossreactivity: Human, mouse and rat.