SAPK2, p38 (Stress-activated Protein Kinase 2, HOG1)

p38 (HOG1), also known as SAPK-2, reactivating kinase or cytokine-suppressive binding protein, is a mouse 38 kD serine threonine kinase that belongs to the mitogen-activated protein kinase family (1). p38 is identified as one of the major tyrosine-phosphorylated proteins in mouse monocyte cell lines upon lipopolysachharide (LPS) treatment (2). Human homolog of p38 binds to cytokines suppressive anti-inflammatory drugs (CSAIDs), an anti-inflammatory drug that inhibits IL-1 and TNF-a synthesis (1). HOG1, the yeast homolog of p38, is stimulated by osmotic shock (3). p38 is activated by MAPK kinase MKK3 and Sek1/MKK4, the latter kinase is also involved in the activation of the stress-activated protein kinase pathway (4). In LPS-treated monocytic cells, the activity of a transcription activators called myocyte-enhancer factor 2 (MEF2) is increased through p38-catalysed phosphorylation, which results in increased c-jun gene transcription (5). p38 also activates MAPKAP kinase 2 in IL-1-stimulated cells (6).\n \nApplications: \nWestern Blot (ECL): 1:10,000 \nImmunoprecipitation: 1:500 \nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nHeLa Cell Lysate, \nMouse Brain Tissue Extract \nRat Brain Tissue Extract

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SPECIFICATIONS

Catalog Number

S0096-24

Size

25ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, GP, NHP, Prc, Rabbit, Sheep

Form

Serum

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Lee, J. C., et al. (1994) Nature 372: 739-746. \n2. Han, J., Bibbs, L., and Ulevitch, R. J. (1994) Science 265: 808-811. \n3. Brewster, J. L. (1993) Science 259: 1760-1763. \n4. Derijard, B., et al. (1995) Science 267: 682-685. \n5. Han, J. et al. (1997) Nature 386: 296-299. \n6. Freshney, N.W. et al (1995) Cell 78: 1039-1049. \n

Additional Info

This antibody detects an ~43kD protein, corresponding to the apparent molecular mass of p38 (HOG1) on SDS-PAGE immunoblots, in samples from human, monkey, mouse, rat, bovine, rabbit, porcine, canine, hamster, chicken, sheep and guinea pig.