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SAPK4, p38 delta, active, Recombinant, Human (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta)

Cat no: S0096-52

SAPK4, p38 delta, active, Recombinant, Human (Stress-activated Protein Kinase 4, Mitogen-activated Protein Kinase 13, MAP Kinase 13, MAPK 13, MAPK13, PRKM13, Mitogen-activated Protein Kinase p38 delta, MAP Kinase p38 delta, p38delta)

Recombinant GST fusion protein fused to full length human SAPK4, expressed in E. coli.\n\nHuman stress-activated protein kinase-4 (SAPK4) is a novel MAP Kinase family member whose amino acid sequence is 60% identical to that of the other three SAP Kinases which contain a TGY motif in their activation domain. Recent results suggest that cellular functions attributed to SAPK1 and/or SAPK2 may be mediated by either SAPK3 or SAPK4. \n\nSpecific Activity: ~100U/mg where 1unit pf p38 delta/SAPK4 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein/minute at 30 degrees C with a final ATP of 100uM.\n\nKinase Assay: Enzyme was tested in a functional kinase assay by using 10-100ng of active SAPK4 to phosphorylate MBP in vitro. \n\nStorage and Stability: Stable for 6 months at -20 degrees C from date of shipment.

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SPECIFICATIONS

Catalog Number

S0096-52

Size

10ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol, and 50% glycerol.

Purity

Purified to (same/more than)50% by affinity chromatography on glutathione Sepharose beads after activation with SKK3/MEK6.

References

Goedert, M., et al., EMBO Journal: 16: 3563

Alternative Names

EC=2.7.11.24

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