Saporin (Saponaria officinalis, Common Soapwort)

Saporin is a ribosome-inactivating protein (RIP) of type I. This monomeric RNA N-glycosidase purified from seeds of the plant Saponaria officinalis also known as Soapwort, is capable of specific depurination of eukaryotic ribosomes thus arresting protein synthesis. No ligand has been identified in saporin hence its inability to transverse the cell membrane. Due to its toxicity and stability of the structure, saporin has proven extremely useful for construction of immunotoxins. The expected molecular weight of the purified saporin is 29.5kD.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0097-18A

Size

100ul

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

P Type

Pab

Purity

Serum

Isotype

IgG

References

General References:\n1. Hill, W. E., A. Dahlberg, et al., Eds. (1990). The Ribosome. Washington, D.C., American Society of Microbiology.\n2. Santache, S., A. Bellelli, et al. (1997). \"The unusual stability of saporin, a candidate for the synthesis of immunotoxins.\" Biochem.Biophys.Res.Commun. 234: 129-132.\n3. Stirpe, F., L. Barbieri, et al. (1992). \"Ribosome-inactivating proteins from plants: present status and future prospects.\" Bio/Technology 10: 405-412.\n4. Stirpe, F., A. Gasper-Campani, et al. (1983). \"Ribosome-inactivating proteins from the seeds of Saponaria officinalis L. (soapwort) of Agrostemma githago L. (corn cockle) and of Asparagus officinalis (asparagus) and from the latex of Hura crepitans L. (sandbox tree).\" Biochemical Journal 216: 617-625.\n5. Wenk, G. L., J. D. Stoehr, et al. (1994). \"Behavioral, biochemical, histological, and electrophysiological effects of 192 IgG-saporin injections into the basal forebrain of rats.\" Journal of Neuroscience 14: 5986-5995.\n\nhttp://en.wikipedia.org/wiki/Saporin

Additional Info

Recognizes Saporin. Confirmed to react with purified Saporin.