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SAR1A (GTP-binding Protein SAR1a, COPII-associated Small GTPase, SARA1, SARA, SAR1)

SAR1A (GTP-binding Protein SAR1a, COPII-associated Small GTPase, SARA1, SARA, SAR1)

Cat no: S0098-07H

Supplier: United States Biological
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Involved in transport from the endoplasmic reticulum to the Golgi apparatus. Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.3ug/ml, observed in lysates of NIH3T3 on ~23kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: S0098-07H
Reactivities: Human, Mouse, Rat
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Bi X, Mancias JD, Goldberg J. Insights into COPII coat nucleation from the structure of Sec23.Sar1 complexed with the active fragment of Sec31. with the active fragment of Sec31. Dev Cell. 2007 Nov;13(5):635-45.
Additional info: Recognizes mouse SAR1A. Species sequence homology: Human and rat.

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