Sarcalumenin

Sarcalumenin is a 160kD acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner.The gene encoding sarcalumenin also encodes an alternate splice variant, a 53kD glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration. \n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500; Immunofluorescence staining of sarcalumenin and its splice variant in rabbit skeletal muscle results in staining that co-localizes with calcium ATPase in the longitudinal sarcoplasmic reticulum (SR) and the terminal cisternae.\nWestern Blot: 1:1000; Detects a 53kD and a 160kD protein representing the glycoprotein and sarcalumenin, respectively, in rabbit skeletal muscle extracts. \n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S0150

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Mouse, Can, GP, Rabbit

Form

Supplied as a liquid in PBS with 0.05% sodium azide.

P Type

Mab

Purity

Ascites.

Isotype

IgG1

References

Ohlendieck K et al. Analysis of excitation-contraction-coupling components in chronically stimulated canine skeletal muscle. Eur J Biochem 202 :739-47 (1991). PubMed: 1662614 \n\nLeberer E et al. Purification, calcium binding properties, and ultrastructural localization of the 53,000- and 160,000 (sarcalumenin)-dalton glycoproteins of the sarcoplasmic reticulum. J Biol Chem 265 :10118-24 (1990). PubMed: 2112542 \n\nMovsesian MA et al. Identification and characterization of proteins in sarcoplasmic reticulum from normal and failing human left ventricles. J Mol Cell Cardiol 22 :1477-85 (1990). PubMed: 2089160

Additional Info

Specifically detects sarcalumenin and the 53kD glycoprotein splice variant. Species Crossreactivity: Mouse, Canine and Guinea pig.