Sarcoglycan, delta

Delta-sarcoglycan is one of six known sarcoglycans, which are type I (alpha and epsilon) or type II (beta, gamma, delta or zeta) transmembrane glycoproteins. Sarcoglycans associate in different combinations in a tissue-specific manner, to form heterotetrameric, pentameric and hexameric membrane glycoprotein complexes (SGC). SGC interacts laterally with the dystroglycan complex, and both complexes are components of the dystrophin-associated glycoprotein complex (DGC), which forms a link between the cytoskeleton and the extracellular matrix. Mutations in sarcoglycans are associated with recessive autosomally inherited muscular dystrophy.\n\nApplications: \nSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1.0ug/ml; Detection limit is ~0.3ng/well\nWestern Blot: 0.1-0.2ug/ml; Detection limit is ~5ng/lane (non-reducing) and 1ng/lane (reducing)\nImmunohistochemistry: 10ug/ml; Detects delta-Sarcoglycan in human fetal heart tissue sections. Sections were fixed with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted S0160 overnight at 4 degrees C followed by Rhodamine Red-coupled anti-goat IgG at RT in the dark for 1 hr. Between each step, cells were washed with PBS, 1% BSA.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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