Sarcoplasmic Reticulum, Junctional

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR?s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyRs), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (95kD) transmits electrochemical signals to the SR through direct interaction with both DHPRs and RyRs. Another skeletal muscle specific 90kD protein has also been localized to the this junction. This protein is phosphorylated by an intrinsic protein kinase in triads suggesting that it may play an important regulatory or structural role in the skeletal muscle triad junction. \n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:250; Immunofluorescence staining of junctional sarcoplasmic reticulum results in increased intensity in fast (Type II) skeletal muscle over slow (Type I) skeletal muscle \nWestern Blot: 1:1500; Detects an ~90kD protein representing junctional SR in rabbit skeletal muscle extracts. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0170

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Mouse, Can, GP, Rabbit

Form

Supplied as a liquid in PBS with 0.05% sodium azide.

P Type

Mab

Purity

Ascites.

Isotype

IgG1

References

Guo W et al. Characterization and ultrastructural localization of a novel 90-kDa protein unique to skeletal muscle junctional sarcoplasmic reticulum. J Biol Chem 269 :28359-65 (1994). PubMed: 7961775

Additional Info

Detects Junctional Sarcoplasmic Reticulum in skeletal muscle. It does not crossreact with cardiac muscle. Crossreacts strongly with Rabbit and Guinea pig, moderately with canine and Rat and weakly with Mouse.