SARS, BioAssay(TM) ELISA Kit (Severe Acute Respiratory Syndrome)

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral S (Spike) protein, a glycosylated 139kD protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. The M protein (Membrane protein, Matrix protein) is another major structural viral protein. It is an integral membrane protein involved in the budding of the viral particles and interacts with S protein and the nucleocapsid protein. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers, which may explain its role in viral budding and virion envelope morphogenesis. ACE2, the SARS receptor, normally plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis.\n\nIntended Use:\nEach antibody will detect 10ng of its corresponding peptide. ACE2, ID can also be used in immunoblot and immunohistochemistry applications.\n\nSpecificity:\nRabbit polyclonal antibodies were raised against peptide sequences corresponding to each of the target proteins. These polyclonal antibodies can be used for detection of SARS Envelope, Matrix or Spike proteins in bodily fluid or tissue by ELISA. Immunogenic peptides are provided as positive controls and to determine protein concentration. \n\nKit Components:\nACE2, ID, 1x25ug\nSARS Envelope, CT, 1x25ug\nSARS Matrix, CT, 1x25ug\nSARS Spike, CT, 1x25ug\nSARS Envelope Peptide, CT, 1x25ug\nSARS Matrix Peptide, CT, 1x25ug\nSARS Spike Peptide, CT, 1x25ug\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S0098-48

Size

1 Set

Applications

IHC, WB

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

References

1. Marra, MA. et al. Science 300, 1399 (2003). 2. Rota, PA. et al. Science 300, 1394 (2003). 3. Navas-Nartin, SR. et al. J. Neurovirol. 10, 75 (2004). 4. Li, W. et al. Nature 426, 450 (2003).