SARS E Protein (Severe Acute Respiratory Syndrome)

A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome) (1-2). Coronaviruses are a major cause of upper respiratory diseases in humans (3). The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. The SARS E protein contains a short palindromic transmembrane helical hairpin that seems to deform lipid bilayers (4), which may explain its role in viral budding and virion envelope morphogenesis (5). \n\nApplications:\nCan be used for the detection of SARS E protein in ELISA. It will detect 10ng of free peptide at 1mg/ml.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0097-99

Size

100ug

Applications

ELISA

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Marra MA, Jones SJ, Astell CR, et al. The Genome sequence of the SARS-associated corona virus. Science 2003;300:1399-404. 2. Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003;300:1394-9. 3. Navas-Nartin SR and Weiss S. Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol. 2004;10:75-85. 4. Arbely E, Khattari Z, Brotons G, et al. A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E protein. J Mol. Biol. 2004;3414:769-79. 5. Maeda J, Maeda A, and Makino S. Release of coronavirus E protein in membrane vesicles from virusinfected cells and E protein-expressing cells. Virology 1999;263:265-72.

Additional Info

Recognizes SARS E protein.