SARS Nucleoprotein (Severe Acute Respiratory Syndrome)

The Coronaviridae virus family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The N protein (nucleoprotein) is one of the major structural proteins in a viral particle, playing a critical role in the transcription regulation of the genomic RNA and other viral proteins. It might also be involved in the virulence and virus-specific post-translational modifications. The N protein is composed of 422 amino acids (a.a.) with an estimated molecular weight of 46.03 kD. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The N protein contains multiple candidate phosphorylation sites, suggesting that it may be related to important functions, such as\nRNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus. \n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nImmunofluorescence: 1-5ug/ml\nWestern Blot: 1-5ug/ml\nELISA: 0.1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n

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SPECIFICATIONS

Catalog Number

S0098-19

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A chromatography.

Isotype

IgG2a

Additional Info

Recogizes the human SARS-CoV nucleoprotein. On Western blots, it identifies the target band at ~50kD. Does not crossreact with the nucleoprotein of 229E-CoV or DC43-CoV. Reactivity has also been confirmed with SARS infected MDCK cells by immunofluorscence.