SARS, Spike Protein (Severe Acute Respiratory Syndrome, SARS-CoV, SARS Associated Coronavirus)

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kD protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients. \n\nApplications: \nSuitable for the detection of SARS Spike protein in ELISA. It will detect 50 ngs of free peptide at 0.5-1ug/ml. \n\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n

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SPECIFICATIONS

Catalog Number

S0098-27

Size

100ug

Applications

ELISA

Hosts

Rabbit

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, et al. Science. 300(5624):1399-1404 (2003). \n\n2. Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, et al. Science. 300(5624):1394-1399 (2003). \n\n3. Krokhin O, Li Y, Andonov A, Feldmann H, Flick R, Jones S, Stroeher U. Mol Cell Proteomics. May;2(5):346-356 (2003). \n\n4. Snijder,E.J., Bredenbeek,P.J., Dobbe,J.C., Thiel,V., Ziebuhr,J., et al. J. Mol. Biol. 331 (5), 991-1004 (2003).

Additional Info

Recognizes SARS, Spike Protein (Severe Acute Respiratory Syndrome)