SATB2, CT (DNA-binding Protein SATB2, Special AT-rich Sequence-binding Protein 2, KIAA1034, FLJ21474, FLJ32076, MGC119474, MGC119477)

Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development. At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2-4ug/ml\nImmunofluorescence: 20ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nA20 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

146289

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Dickinson LA and Kohwi-Shigematsu T. Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol. Cell. Biol. 1995; 15:45665.\n2. Szemes M, Gyorgy A, Pawaletz C, et al. Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain. Neurochem. Res. 2006; 31:237-46. 3. Dobreva G, Chahour M, Dautzenberg M, et al. SATB2 is a multifunctional determinant of craniofacial patterning and osteoblast differentiation. Cell 2006; 125:971-86. 4. Gyorgy AB, Szemes M, de Jaun Romero C, et al. SATB2 interacts with chromatin-remodeling molecules in differentiating cortical neurons. Eur. J. Neurosci. 2008; 27:865-73.

Additional Info

Recognizes human SATB2. Species Crossreactivity: mouse and rat.