Scavenger Receptor A Type I/II (CD204, SRA) (FITC)

CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion.\n\nApplications:\nSuitable for use in Flow Cytometry. Not suitable for Immunohistochemistry, paraffin or resin. Other applications not tested. \n\nRecommended Dilutions: \nFlow Cytometry: 10ul labels 10e6 cells in 100ul.\nOptimal dilution determined by the researcher. \n\nRecommended Negative Control:\nI1904-84J1: IgG2b Negative Control (FITC)\n\nHybridoma: \nSpleen cells from immunized AO rats were fused with cells of the Y3 rat myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0099-06

Size

50ug

Applications

FC

Hosts

Rat

Reactivities

Mouse, Prc

Form

Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Fraser, I.P. et al. (1993). Nature 364: 343-346. 2. de Villiers, W.J.S. et al. (1994). J. Exp. Med. 180: 705-709. 3. Hughes, D.A. et al. (1995). . Eur. J. Immunol. 25: 466-473. 4. Bell, M.D. et al. (1994). J. Neurocytol. 23: 605-613. 5. Hughes, D.A. et al. (1994). . Imm. Letts. 43: 7-14. 6 Rosen, H. and Hughes, D.A. (1995). Assays of Myeloid Cell Function: Migration and adhesion in vivo. Weir Handbook of Experimental Immunology. London, Blackwell Scientific Publications. 5th, ed. In Press. 7. Daugherty, A. et al. (2000). Polymorphism of class A scavenger receptors in C57BL/6 mice. J. Lipid Res. 41: 1568 - 1577

Additional Info

Recognizes the mouse scavenger receptor class A (SRA), type I and II, also known as CD204. Recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice and is therefore unsuitable for use with the C57BL/6 mouse strain. \nSpecies Crossreactivity: porcine