Scavenger Receptor BII (SR-BII)

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism. HDL plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs mediating selective uptake of HDL cholesteryl ester; with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI. SR-BII expression found in liver, adrenal glands, and testes. The role of SR-BII is not completely clear. Research suggests that it may be a functional HDL receptor. SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells (1-6).\n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications not tested\n\nRecommended Dilution:\nWestern Blot: 1:500. A band of ~82kD is seen in tissues that express SR-BII such as liver, tested and adrenal glands.\nImmunofluorescence: 1:250-1:500\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0099-010

Size

100ul

Applications

ELISA, IF, WB

Hosts

Rabbit

Reactivities

Mouse, Rat, Bov

Form

Supplied as a liquid, serum, 0.05% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. N.R. Webb, et al. SR-BII, an Isoform of the Scavenger Receptor BI Containing an Alternate Cytoplasmic Tail, Mediates Lipid Transfer between High Density Lipoprotein and Cells. J. of Biol. Chem. 273(24): 15241-15248 1998. 2. N.R. Webb, et al. Alternative forms of the scavenger receptor B1 (SR-B1). J. Lipid Research 38: 1490-1495, 1997. 3. M. Krieger. The \"best\" of cholesterols the \"worst\" of cholesterols: A tale of two receptors. PNAS 95: 4077-4080, 1988. 4. S. Acton, et al. Identification of Scavenger Receptor SR-BI as a High Density Lipoprotein Receptor. Science 271: 518-520, 1996. 5. Rigotti, et al. A targeted mutation in the murine gene encoding the high density lipoprotein (HDL) receptor scavenger receptor class B type I reveals its key role in HDL metabolism. PNAS 94: 12610-12615, 1997. 6. R.E. Temel, et al. Scavenger receptor class B, type I (SR-BI) is the major route for the delivery of high density lipoprotein cholesterol to the steroidgenic pathway in cultured mouse adrenocortical cells. PNAS 94: 13600-13605, 1997.

Additional Info

Specific for SR-BII. Species crossreactivity: Mouse, rat, bovine and hamster.