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sCD40 Ligand, Recombinant, Mouse (soluble CD40 Ligand, TNFSF5, TRAP, CD154, gp39, T-BAM)

Cat no: 143493

sCD40 Ligand, Recombinant, Mouse (soluble CD40 Ligand, TNFSF5, TRAP, CD154, gp39, T-BAM)

CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells.Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching.The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes.Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE.The soluble form of CD40L is an 18kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L.Recombinant murine CD40L is a soluble 16.4kD protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.\n\nBiological Activity:\nDetermined by its ability to induce TNF-alpha; and MIP-1alpha; production by murine splenocytes. The expected ED50 for this effect is ~0.1ug/ml.\n\nAA Sequence:\nMQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL\n\nCrossreactivity:\nHuman, Rat\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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SPECIFICATIONS

Catalog Number

143493

Size

5ug

Form

Supplied as a lyophilized powder.

Purity

(same/more than)98% by SDS-PAGE gel and HPLC analyses.

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