sCD40L BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human soluble CD40L in cell culture supernates, serum and plasma (heparin, EDTA).\n\nCD40 ligand(CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 15 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP29965\n\nEntrez Gene ID:\n959\n\nKit Components:\n96-well plate pre-coated with anti-sCD40L, Human\nLyophilized recombinant sCD40L, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144031

Size

96Tests

References

1. Allen,R.C.;Armitage,R.J.;Conley,M.E.;Rosenblatt,H.;Jenkins,N.A.;Copeland,N.G.;Bedell,M.A.;Edelhoff,S.;Disteche,C.M.;Simoneaux,D.K.;Fanslow,W.C.;Belmont,J.;Spriggs,M.K.:CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome. Science 259: 990-993, 1993.\n2. Cipollone,F.;Ferri,C.;Desideri,G.;Paloscia,L.;Materazzo,G.;Mascellanti,M.;Fazia,M.;Iezzi,A.;Cuccurullo,C.;Pini,B.;Bucci,M.;Santucci,A.;Cuccurullo,F.;Mezzetti,A.:Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty. Circulation 108: 2776-2782, 2003.\n