SCFB-TrCP1 Complex, Active, Recombinant, Human (SCF-B-TrCP,SCFFWD10)

The SCF complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFBTrCP1 mediates the ubiquitination and subsequent proteasomal degradation of a variety of proteins involved in various cellular processes, these include IkBa, an inhibitor of the transcription factor NFkB and B-catenin which is involved in the Wnt signalling pathway. A number of cancers, including those in breast, colon and pancreatic tissue have been associated with the deregulation of SCFBTrCP1.\n\nSource: \nRecombinant corresponding to complex of N-terminal GST- tagged, recombinant human bTrCP1 aa53-end, N-terminal GST-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells.\n\nMolecular Weight: \nbTrCP1: ~91kD, Skp1: ~46kD, Cul1: ~93kD, Rbx1: ~12kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029605

Size

10ug

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris/HCl pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol.

Purity

~92% (SDS-PAGE)

References

1. Willems A. R. et al. A Hitchhiker's Guide to the Cullin Ubiquitin Ligases: SCF and its Kin. Biochimica et Biophysica Acta., 1695: 133-170, 2004. 2. Frescas D. and Pagano M. Deregulated Proteolysis by the F-box Proteins SKP2 and b-TrCP: tipping the scales of cancer. Nat Rev Cancer, 8: 438-449, 2008. 3. Ougolkov A. et al., Associations Among b-TrCP, an E3 Ubiquitin Ligase Receptor, b-catenin, and NFkb in Colorectal Cancer. J Natl Cancer Inst. 96: 1161-1170, 2004. 4. Kitagawa M. et al., An F-box protein, FWD1, Mediates Ubiquitin Dependent Proteolysis of b-catenin. The EMBO Journal, 18: 2401-2410, 1999. 5. Strack P. et al., SCFb-TRCP and Phosphorylation Dependent Ubiquitination of IkBa Catalysed by Ubc3 and Ubc4. Oncogene, 19: 3529-3536, 2000.