SCFFbw7 Complex, Active, Recombinant, Human (SCFFbxw7, SCFhCdc4)

The SCF complexes represent the largest family of ubiquitin-protein ligases and mediate the ubiquitination of a broad spectrum of regulatory and signalling proteins in diverse cellular pathways. The SCF consists of three invariant components, Skp1, Cul1, and Rbx1 and an interchangeable subunit, an F-box protein which is responsible for recruiting specific substrates to be ubiquitinated by the SCF. SCFFbw7 mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell division, growth and differentiation, including Cyclin E1, c-Myc, Notch and c-Jun. Mutations have been found in the Fbw7 subunit of SCFFbw7 in various types of human cancers, therefore Fbw7 is considered to be a potent tumour suppressor.\n\nSource: \nRecombinant corresponding to complex of N-terminal GST-tagged, recombinant human Fbw7 full length, N-terminal 6His-tagged, recombinant human Skp1 full length, N-terminal 6His-tagged, recombinant human Cul1 full length and untagged recombinant human Rbx1 full length, co-expressed by baculovirus in Sf21 insect cells.\n\nMolecular Weight: \nFbw7: ~107kD, Skp1: ~21kD, Cul1: ~93kD, Rbx1: ~12kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029606

Size

10ug

Form

Supplied as a liquid in 50mM Tris/HCl pH7.5, 300mM sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Purity

~93% (SDS-PAGE, Coomassie blue staining)

References

1. Willems A. R. et al. A Hitchhiker's Guide to the Cullin Ubiquitin Ligases: SCF and its Kin. Biochimica et Biophysica Acta., 1695: 133-170, 2004. 2. Minella A.C. and Clurman B.E. Mechanisms of Tumor Suppression by the SCFFbw7. Cell Cycle 4: 1356-1359, 2005. 3. Welcker M. et al., The Fbw7 Tumor Suppressor Regulates Glycogen Synthase Kinase 3 Phosphorylation- Dependent c-Myc Protein Degradation. PNAS, 101: 9085-9090, 2004. 4. Koepp D.M. et al., Phosphorylation-Dependent Ubiquitination of Cyclin E by the SCFFbw7 Ubiquitin Ligase. Science, 294: 173-177, 2001.