Scribble (Scribbled homolog (Drosophila), CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB1, Vartul, VARTUL)

Scribble (Scrib) was originally identified in a genetic screen in Drosophila along with cell polarity determinants Discs Large (Dlg) and Lethal giant larvae (Lgl). Drosophila mutants homozygous for these genes share similar phenotypes, including the loss of apicobasal cell polarity and neoplastic tissue overgrowth. These phenotypic similarities suggest that these three proteins function in a common pathway important for establishing and maintaining apicobasal polarity in epithelial cells (1,2). Scribble contains many leucine-rich repeats and PDZ domains important for localizing scribble to adherens junctions and basolateral regions of mammalian epithelial cells (3). Scribble reportedly binds b-catenin, APC, E-cadherin and the E6 protein from high-risk virus type of HPV through a short motif important for E6-induced cell transformation (4-8). Overexpression of scribble inhibits transformation of rodent epithelial cells by HPV E6/7 proteins (8). \n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% \n Tween-20 at 4 degrees C with gentle shaking, overnight. \nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0820

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Bilder, D. and Perrimon, N. (2000) Nature 403, 676-80, 2. Bilder, D. et al. (2000) Science 289, 113-6, 3. Humbert, P.O. et al. (2008) Oncogene 27, 6888-907, 4. Sun, Y. et al. (2009) Mol Biol Cell 20, 3390-400, 5. Qin, Y. et al. (2005) J Cell Biol 171, 1061-71, 6. Navarro, C. et al. (2005) Oncogene 24, 4330-9, 7. Takizawa, S. et al. (2006) Genes Cells 11, 453-64, 8. Nguyen, M.L. et al. (2003) J Virol 77, 6957-64.

Additional Info

Recognizes human Scribble. Species crossreactivity: monkey.