SDF-1b (Stromal Cell-derived Factor-b, Pre-B-cell Growth-stimulating Factor, PBSF)

SDF-1b (stromal cell-derived factor-b), also known as pre-B-cell growth-stimulating factor (PBSF), is a 74-amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. Targeted deletion of SDF-1 gene resulted in defects of B-cell lymphopoiesis and bone marrow myelopoiesis in mice. SDF-1 has been shown to be chemotactic for lymphocytes. In addition, SDF-1 was recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV-1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV-1 entry.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nFlow Cytometry: 1:100\nImmunohistochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S7975-63

Size

100ug

Applications

FC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid, 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Tashiro, K., et al., Science 261: 600-603 (1993). 2. Nagasawa, T., et al., Proc. Natl. Acad. Sci. USA 91: 2305-2309 (1994). 3. Nagasawa, T., et al., Nature 328: 635-638 (1996). 4. Bleul, C.C., et al., J. Exp. Med. 184: 1101-1109 (1996). 5. Bleul, C.C., et al., Nature 382: 829-833 (1996). 6. Oberlin, E., et al., Nature 382: 833-835 (1996). 7. Reissa, K., et al., Neuroscience 115(1): 295-305 (2002). 8. Renata, S., et al., Blood 106(7): 2259-2268 (2005).

Additional Info

Recognizes mouse SDF-1b. Species Crossreactivity: rat.