SDHA (Succinate Dehydrogenase [ubiquinone] Flavoprotein Subunit, Mitochondrial, Flavoprotein Subunit of Complex II, Fp, SDH2, SDHF)

Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Defects in SDHA cause complex II deficiency (2). In addition, reduction of SDHA has been observed in the striatum of patients with Huntington's Disease (3). Reduction of SDHB, SDHC, and SDHD are often observed in paragangliomas and phenochromocytomas (4,5).\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence (IF-IC): 1:50\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0553-24A

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1.Oyedotun, K.S. and Lemire, B.D. (2004) J Biol Chem 279, 9424-31. 2.Bourgeron, T. et al. (1995) Nat Genet 11, 144-9. 3.Benchoua, A. et al. (2006) Mol Biol Cell 17, 1652-63. 4.Baysal, B.E. et al. (2000) Science 287, 848-51. 5.Feichtinger, R.G. et al. (2010) BMC Cancer 10, 149.

Additional Info

Recognizes endogenous levels of total human SDHA. Species Crossreactivity: Monkey, mouse and rat.