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SDR16C5, Recombinant, Human, aa32-269, His-Tag (Short Chain Dehydrogenase/reductase Family 16C, RDH#2, RDH-E2, RDHE2)

Cat no: 137202

SDR16C5, Recombinant, Human, aa32-269, His-Tag (Short Chain Dehydrogenase/reductase Family 16C, RDH#2, RDH-E2, RDHE2)

Short chain dehydrogenase/reductase family 16C, member5, also known as SDR16C5, is active in both the oxidative and reductive directions. This protein oxidizes all-trans-retinol in all-trans-retinaldehyde. No activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH. \n\nSource: \nRecombinant protein corresponding to human SDR16C5, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~28.3kD (261aa) (MALDI-TOF)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSPKPRKNV AGEIVLITGA GSGLGRLLAL QFARLGSVLV LWDINKEGNE ETCKMAREAG ATRVHAYTCD CSQKEGVYRV ADQVKKEVGD VSILINNAGI VTGKKFLDCP DELMEKSFDV NFKAHLWTYK AFLPAMIAND HGHLVCISSS AGLSGVNGLA DYCASKFAAF GFAESVFVET FVQKQKGIKT TIVCPFFIKT GMFEGCTTGC PSLLPILEPK YAVEKIVEAI LQEKMYLYMP K\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137202

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M NaCl, 10% glycerol, 1mM DTT.

Purity

~90% (SDS-PAGE)

References

1. Matsuzaka Y., et al. (2002) Biochem. Biophys. Res. Commun. 297:1171-1180. 2. Lee S.-A., et al. (2009) Chem. Biol. Interact. 178:182-187.

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