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Secretase, alpha FRET Peptide, MCA-HQKLVFFA (K-DNP)

Cat no: S0618

Secretase, alpha FRET Peptide, MCA-HQKLVFFA (K-DNP)

Fluorescent resonance energy transfer (FRET) peptide for detecting a-secretase. Labeled with methoxycoumarin acetic acid (MCA) and dinitrophenyl (DNP).\n\nSequence (linear): MCA-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-(Lys-DNP)-OH\n\nAmino Acid Analysis: Confirms expected sequence\nPeptide Content: 80.0

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SPECIFICATIONS

Catalog Number

S0618

Size

1mg

Form

Supplied as a lyophilized powder.

Purity

~90% by HPLC and MS analysis

References

References using FRET MCA-peptide-DNP\nNishikata M, Suzuki K, Yoshimura Y, Deyama Y, Matsumoto A. A phosphotyrosine-containing quenched fluorogenic peptide as a novel substrate for protein tyrosine phosphatases. Biochem J 1999 Oct 15;343 Pt 2: 385-91.\nNagase H, Fields CG, Fields GB. Design and characterization of a fluorogenic substrate selectively hydrolyzed by stromelysin 1 (matrix metalloproteinase-3). J Biol Chem 1994 Aug 19;269(33): 20952-7.\nAbramson SR, Conner GE, Nagase H, Neuhaus I, Woessner JF Jr. Characterization of rat uterine matrilysin and its cDNA. Relationship to human pump-1 and activation of procollagenases. J Biol Chem 1995 Jul 7;270(27): 16016-22.\nKnight CG, Willenbrock F, Murphy G. A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases. FEBS Lett 1992 Jan 27;296(3): 263-6

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