Secretin, Human

Human Secretin stimulates the secretion of bicarbonate by the pancreas and inhibits the production of gastrin and acid production in the stomach. It also potentiates the release of digestive enzymes from the pancreas triggered by cholecystokinin. Secretin has a molecular formula of C130H220N44O40 a.a. sequence of -His-Ser-Asp-Gly-Thr-Phe-Thr-Ser-Glu-Leu-Ser-Arg-Leu-Arg- Glu-Gly-Ala-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Gly-Leu-Val-NH2 and having a Mw of 3039.45.\n\nFields of Applications: \nDiagnosis of pancreatic dysfunction. Diagnosis of the presence of a gastrinoma.\n\nDimers and Aggregates: \nLess than 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Secretin.\n\nProtein Content: \nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280 nm.\n2. Analysis by RP-HPLC, using a calibrated solution of Human Secretin as a Reference Standard.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Human Secretin in sterile 18MOmega-cm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.\n\nStability: \nLyophilized Human Secretin although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Human Secretin should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

S0625-03

Size

1mg

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than) 99% as determined by: \n(a) Analysis by RP-HPLC. \n(b) Anion-exchange FPLC. \n(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

References

1. Evaluation of somatostatin inhibitory effect on pancreatic exocrine function using secretin-enhanced dynamic magnetic resonance cholangiopancreatography: a crossover, randomized, double blind, placebo-controlled study.\n\nPancreas 2006 May;32(4):346-50\n\n2. Polar assembly of the type IV pilus secretin in Myxococcus xanthus.\n\nMol Microbiol 2006 Apr;60(1):16-29\n\n3. The role of secretin in the cerebellum.\n\nCerebellum 2006;5(1):43-8\n\n4. Secretin induces neurite outgrowth of PC12 through cAMP-mitogen-activated protein kinase pathway.\n\nExp Mol Med 2006 Feb 28;38(1):85-93\n\n5. Predicted role of secretin and oxytocin in the treatment of behavioral and developmental disorders: implications for autism.\n\nInt Rev Neurobiol 2005;71:273-315\n\n6. Effects of secretin on extracellular GABA and other amino acid concentrations in the rat hippocampus.\n\nInt Rev Neurobiol 2005;71:239-71