SECTM1, Fc Chimera, Recombinant, Human (Secreted and Transmembrane Protein 1, Protein K-12, K12)

SECTM1 (secreted and transmembrane 1), also called K12, is either found as an ~27kD intracellular type I transmembrane protein that shows a perinuclear, Golgi-like staining pattern, or as a 20kD soluble, secreted form. SECTM1 protein expression is detected in some myeloid cells, such as stimulated monocytes, immature monocyte-derived dendritic cells, and granulocytes. In the thymus, it is also expressed by epithelia and fibroblasts. Stimulation with IFNg is often necessary to detect SECTM1 expression, and it is thought to be an interferon earlyresponse gene. Human SECTM1 cDNA encodes 248aa, including a 28aa signal sequence, a 117aa extracellular domain (ECD) with one potential N-linked glycosylation site, a 21aa transmembrane sequence, and an 82aa cytoplasmic sequence. The ECD shows some similarity to Ig domains. The human SECTM1 ECD shares limited (~45-51%) aa sequence identity with mouse SECTM1 (a or b) and rat SECTM1 ECD. SECTM1 from human and mouse each show species specific binding of CD7 and co-stimulate CD4+ and CD8+ T cells through CD7, either alone or through synergy with CD28. Immobilized recombinant human SECTM1/Fc also enhances human NK cell expression of activation markers such as CD25, CD69 and CD54. Source: Recombinant corresponding to aa29-45 from human SECTM1expressed in CHO cells. Molecular Weight: ~39.3kD Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 5x10e4 cells per well are added to recombinant human SECTM1 Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is is typically 1.5-7.5ug/ml. Endotoxin: ~1.0 EU/1ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.