Securin (Esp1-associated Protein, EAP1, Pituitary Tumor-transforming Gene 1 Protein, PTTG1, PTTG, hPTTG, Tumor-transforming Protein 1, TUTR1)

Human securin is also referred to as pituitary tumor- transforming gene-1. It is required for chromosomal stability in human cells for the maintenance of euploidy. Its expression correlates with cell proliferation in a cellcycle dependent manner in normal tissues and several tumor types. Human securin binds to Ku, the controlling subunit of the DNA-dependent protein kinase. This antibody will help detect human securin and further characterize its expression in various tissues.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:25-1:50 for 1 hr. at RT\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nTonsil (cytoplasmic and nuclear staining pattern)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0379-80E

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a,k

References

1.Ishikawa H, Heaney A P, Yu R, et al.. Human pituitary tumor-transforming gene induces angiogenesis. J. Clin. Endocrinol. Metab. 86(2):867-874 (2001). 2.Chein W and Pei L. A novel binding factor facilitates nuclear translocation and transcriptional activation function of the pituitary tumor-transforming gene product. Journal of Biological Chemistry. 275(25):19422-19427 (2000). 3. Heaney A P, Singson R, McCabe C J, et al.. Expression of pituitary-tumour transforming gene in colorectal tumours. Lancet. 355(9205):716-719 (2000). 4.Yanagida M. Cell cycle mechanisms of sister chromatid separation; roles of Cut1/separin and Cut2/securin. Genes Cells. 5(1):1-8 (2000). 5.McCabe C J and Gittoes N J. PTTG-a new pituitary tumour transforming gene. Journal of Endocrinology. 162(2):163-166 (1999).

Additional Info

Recognizes human Securin.

Alternative Names

Pituitary Tumor-transforming 1