SEK1, phosphorylated (Ser257,Thr261) (MKK4, SAPK, Erk Kinase, MKK4, Jun Kinase Kinase, JNKK)

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines. Activation of SEK1 occurs through phosphorylation of serine and threonine residues at positions 257 and 261, respectively, by MEKK. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop. Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses the stress-activated signal transduction.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

J2475-05A

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Dro/Arth

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473 2. Sanchez, I. et al. (1994) Nature 372, 794-798. 3. Yan, M. et al. (1994) Nature 372, 798-800. 4. Kyriakis, J.M. et al. (1994) Nature 369, 156-160. 5. Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

Additional Info

Recognizes endogenous levels of human SEK1/MKK4 only when phosphorylated at serine 257 and threonine 261. Does not crossreact with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3. Species Crossreactivity: mouse, rat and Drosophilia.