Semaphorin 3A, Fc Chimera, Recombinant, Mouse (Semaphorin III, Sema III, SEMA3A, SEMAD)

Semaphorin 3A is one of six Class 3 secreted semaphorins which share ~40-50% aa identity. Class 3 semaphorins are potent chemo- repellents that function in axon and/or vascular guidance during development. The 772aa mouse Sema3C contains a 20aa signal sequence, an ~500aa N-terminal Sema domain that forms a b-propeller structure similar to that found in integrin molecules, a PSI domain, a furin- type cleavage site, an Ig-like domain, and a C-terminal basic domain. Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins. The 95kD mature mouse Sema3A shares at least 95% aa identity with human, rat, equine and canine Sema3A, and 90% and 86% aa identity with chick and zebrafish Sema3A, r espectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors. Sema3A signaling is transduced by plexin A1-4, indirectly via neuropilin-1. Sema3A activity is mediated by small GTPases that influence actin rearrangement and integrin activity. It is important in developmental organization of central and peripheral nerves, including those in heart , lung, kidneys, bones, teeth, and visual and olfactory systems. Gradients of Sema3A repel axons, but attract dendrites. Sema3A affect vasculogenesis by inhibiting integrin function and, with Sema3F, promoting apoptosis of endothelial cells. It is thought to suppress cancer-related angiogenesis. In the immune system, Sema3A influences T cell proliferation, migration, response to activation, and interactions with dendritic cells. It negatively regulates platelet activation. Expression of Sema3A in relevant parts of the nervous system may be increased in Alzheimer's disease, multiple sclerosis, ischemia and schizophrenia.\n\nSource:\nRecombinant corresponding to mouse Semaphorin 3A (Asn21-Lys747: Arg552Ala, Arg554Ala, Arg555Ala, Arg618Ala, Lys619Ala, Lys642Ala, Lys643Ala; Accession #O08665), the sequence IEGRMDP followed by mouse IgG2a (Glu98-Lys330) expressed in CHO cells.\n\nMolecular Weight: 115-120kD (SDS-PAGE, reducing conditions) \n\nBiological Activity:\nMeasured by its ability to cause collapse of chick dorsal root ganglia (DRG) neuron growth cones. The ED50 for this effect is typically 20-60ng/ml in the presence of 10ng/ml recombinant human beta-NGF.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0668-47S

Size

25ug

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with PBS to 100ug/ml.

Purity

(same/more than)90% by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: (same/less than)1EU/ug (LAL)

References

1. Puschel , A. W. et al. (1995) Neuron 14:941. 2. Roth, L. et al. (2009) Cell. Mol. Life Sci. 66:649. 3. Neufeld, G and O. Kessler (2008) Nat. Rev. Cancer 8:632. 4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669. 5. Adams, R. H. et al. (1997) EMBO J. 16:6077. 6. Klosterman, A. et al. (1998) J. Biol . Sci . 273:7326. 7. Lepelletier, Y. et al. (2006) Eur. J. Immunol. 36:1782. 8. Schlomann, U. et al. (2009) J. Cell Sci . 122:2034. 9. Serini, G. et al. (2003) Nature 424:391. 10. Ieda, M. et al. (2007) Nat. Med. 13:604. 11. Chen, G. et al. (2008) Nat. Neurosci. 11:36. 12. Guttmann