The SensiFAST SYBR & Fluorescein One-Step Kit has been developed for fast RT-qPCR and has been validated on several BioRad real-time PCR instruments. The SensiFAST SYBR & Fluorescein One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR & Fluorescein One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR (fig. 1). The SensiFAST SYBR & Fluorescein One-Step Kit consists of a 2x SensiFAST SYBR One-Step mix, which contains fluorescein for optional use, separate reverse transcriptase and RiboSafe RNase Inhibitor.
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