The SensiFAST(TM) SYBR No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR. The SensiFAST SYBR No-ROX One-Step Kit consists of a 2x SensiFAST SYBR One-Step mix, as well as separate reverse transcriptase and RiboSafe RNase Inhibitor.