The SensiFAST SYBR & Fluorescein Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive real-time PCR (fig. 1 and 2). SensiFAST SYBR & Fluorescein Kit has been optimized for use in SYBR Green-based real-time PCR. The SensiFAST SYBR & Fluorescein Kit contains premixed Fluorescein for optional use.