High Resolution Meltcurve (HRM) analysis characterizes nucleic acid samples based on their dissociation behavior. It combines the principle of intercalating dyes, meltcurve analyses and the application of specific statistical analyses. HRM uses the fundamental property of the separation of the two strands of DNA with heat (melting), and the monitoring of this melting with a fluorescent dye. The SensiMix HRM Kit employs a 3rd generation saturating dye that does not inhibit PCR at high concentrations and has been validated for use on the Roche LightCycler® 480 and the Corbett Rotor-Gene(TM) 6000.
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