Sentrin 1, Recombinant, Human (Small Ubiquitin-related Modifier 1, SUMO 1, SMT3C)

Active human SUMO-I (the 1-97 amino acid region of the Ubiquitin-like protein SMT3C precursor also named Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, Ubiquitin-related protein SUMO-I, GAP modifying protein-I, GMP-I, and Sentrin-I. Human SUMO-1 (small ubiquitin-like modifier) is an 11kD protein that shares 18% sequence homology with ubiquitin. Smt3p, which is 48% identical to human SUMO-1, has been identified in yeast. SUMO-1/Smt3p is activated by a heterodimeric E1 enzyme in both the yeast and mammalian systems It has been shown that SUMO-1 can be conjugated to a number of proteins such as PML, RanGAP1, I B, FAS/apolipoprotein-1, p53, RAD51 and RAD52.\n\nRecombinant Human SUMO1 is a single, non-glycosylated, polypeptide chain having a molecular mass of 20kD as seen on SDS-PAGE.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0750-01F

Size

20ug

Form

Supplied as a lyophilized powder. No additives.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. The small ubiquitin-like modifier (SUMO) is required for gonadal and uterine-vulval morphogenesis in Caenorhabditis elegans. Broday L, Kolotuev I, Bhoumik A, Ronai Z, Genes Dev 2004 Oct 1;18(19):2380-91 2. p14 Arf promotes small ubiquitin-like modifier conjugation of Werners helicase. Woods YL, Xirodimas DP, Sparks A, J Biol Chem 2004 Nov 26;279(48):50157-66 3. In vitro modification of human centromere protein CENP-C fragments by small ubiquitin-like modifier (SUMO) protein: definitive identification of the modification sites by tandem mass spectrometry analysis of the isopeptides. Chung TL, Hsiao HH, Shia HL, Khoo KH, J Biol Chem 2004 Sep 17;279(38):39653-62 4. Small ubiquitin-like modifier 1 (SUMO-1) modification of the synergy control motif of Ad4 binding protein/steroidogenic factor 1 (Ad4BP/SF-1) regulates synergistic transcription between Ad4BP/SF-1 and Sox9. Komatsu T, Mizusaki H, Ogawa H, Nakayama KI, Mol Endocrinol 2004 Oct;18(10):2451-62 5. Differential effect of small ubiquitin-like modifier (SUMO)-ylation of the androgen receptor in the control of cooperativity on selective versus canonical response elements. Callewaert L, Verrijdt G, Claessens F, Mol Endocrinol 2004 Jun;18(6):1438-49 6. Association between promyelocyte protein and small ubiquitin-like modifier protein and the progression of cervical neoplasia. Szendefi M, Walt H, Caduff R, Obstet Gynecol 2003 Dec;102(6):1269-77.