Seprase/FAP BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human Seprase/FAP in cell culture supernates, serum and plasma (heparin, EDTA).\n\nFAP (Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ12884\n\nEntrez Gene ID:\n2191\n\nKit Components:\n96-well plate pre-coated with anti-Seprase/FAP, Human\nLyophilized recombinant Seprase/FAP, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144037

Size

96Tests

References

1. Goldstein, L. A., Ghersi, G., Pineiro-Sanchez, M. L., Salamone, M., Yeh, Y., Flessate, D., Chen, W.-T. Molecular cloning of seprase: a serine integral membrane protease from human melanoma.Biochim. Biophys. Acta 1361: 11-19, 1997.\n2. Mathew, S., Scanlan, M. J., Mohan Raj, B. K., Murty, V. V. V. S., Garin-Chesa, P., Old, L. J., Rettig, W. J., Chaganti, R. S. K. The gene for fibroblast activation protein alpha (FAP), a putative cell surface-bound serine protease expressed in cancer stroma and wound healing, maps to chromosome band 2q23. Genomics 25: 335-337, 1995.\n3. Scanlan, M. J., Raj, B. K. M., Calvo, B., Garin-Chesa, P., Sanz-Moncasi, M. P., Healey, J. H., Old, L. J., Rettig, W. J. Molecular cloning of fibroblast activation protein alpha, a member of the serine protease family selectively expressed in stromal fibroblasts of epithelial cancers. Proc. Nat. Acad. Sci. 91: 5657-5661, 1994.\n