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SEPT5, Recombinant, Human, aa1-369, His-tag (Septin-5, CDCREL, CDCREL-1, CDCREL1, H5, PNUTL1)

Cat no: S0950-13

SEPT5, Recombinant, Human, aa1-369, His-tag (Septin-5, CDCREL, CDCREL-1, CDCREL1, H5, PNUTL1)

SEPT5, also known as septin-5, belongs to the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Purified by using conventional chromatography.\n\nSource:\nRecombinant corresponding to aa1-369 of human SEPT5, fused to His-tag at N-terminal, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMSTGLRY KSKLATPEDK QDIDKQYVGF ATLPNQVHRK SVKKGFDFTL MVAGESGLGK STLVHSLFLT DLYKDRKLLS AEERISQTVE ILKHTVDIEE KGVKLKLTIV DTPGFGDAVN NTECWKPITD YVDQQFEQYF RDESGLNRKN IQDNRVHCCL YFISPFGHGL RPVDVGFMKA LHEKVNIVPL IAKADCLVPS EIRKLKERIR EEIDKFGIHV YQFPECDSDE DEDFKQQDRE LKESAPFAVI GSNTVVEAKG QRVRGRLYPW GIVEVENQAH CDFVKLRNML IRTHMHDLKD VTCDVHYENY RAHCIQQMTS KLTQDSRMES PIPILPLPTP DAETEKLIRM KDEELRRMQE MLQRMKQQMQ DQ\n\nMolecular Weight: \n~45.2kD (392aa), confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0950-13

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.3M sodium chloride, 1mM DTT, 40% glycerol.

Purity

~90% (SDS-PAGE)

References

Blaeser S., et al. (2002) FEBS Lett. 519:169-172 Hall P.A., et al. (2005) J. Pathol. 206:269-278.

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