Serotonin Receptor 1A (5-HT1A)

The serotonin receptors, also known as 5-hydroxytryptamine receptors or 5-HT receptors, are a group of G protein-coupled receptors (GPCRs) and ligand-gated ion channels (LGICs) found in the central and peripheral nervous systems. They mediate both excitatory and inhibitory neurotransmission. The serotonin receptors are activated by the neurotransmitter serotonin, which acts as their natural ligand.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: Using rat brain extracts of cortex, hypothalamus, midbrain and hindbrain, the antibody specifically labels a single band. Immunolabeling is abolished by pre-adsorption with synthetic rat 5HT transporter (aa579-599).\nImmunohistochemistry (indirect IF): 1:200-1:400 \nImmunohistochemistry (biotin/streptavidin HRP technique): 1:200-1:600 in rat raphe nuclei, hypothalamus, cortex and spinal cord.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1001-15A

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 1% BSA.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Burnet, P.W., et al., Brain Research 676(1): 157-168 (1995). 2. Chalmer, D.T. & Watson, S.J., Brain Research 561: 51-60 (1991). 3. Kia, H.K., et al., J. Comp. Neurology 365a(2) 289-305 (1996). 4. Kongins, P.N., et al., J. Receptor & Signal Transduction Research 15(1-4): 443-455 (1995). 5. Raymond, J.R., et al., Am. J. Physiol. 264: F9-F19 (1993). 6. Wright, D.E., et al., J. Comp. Neurol. 351: 357-373 (1995).

Additional Info

Recognizes rat Serotonin Receptor 1A. Species Crossreactivity: human and mouse.