SERPINB9 (Serine/Cysteine Proteinase Inhibitor Clade B Member 9, Serpin Peptidase Inhibitor Clade B (Ovalbumin) Member 9, Serpin B9, Cytoplasmic Antiproteinase 3, CAP3, CAP-3, Peptidase Inhibitor 9, Proteinase Inhibitor 9, PI9, PI-9)

Applications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Not suitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Detects native SERPINB9 at 4~2kD as well as SERPINB9 complexed to granzyme B at ~67kD. As a positive control for SERPINB9 , YT-Indy cells (a natural killer leukemia cell line) can be used. In other cell lines a non-specific protein band of ~63kD can be detected. \nImmunohistochemistry: 1:100 Can be used on formaldehyde-fixed paraffin-embedded human tissues after pretreatment with 10mM citric acid pH 6.0 for 20 min.\nImmunocytochemistry: Fix cells for 10 min in 10% formalin and then treat them as paraffin sections. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1000-77P2

Size

1ml

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.7% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Bird, C. H., et al. 1998. Selective regulation of apoptosis: the cytotoxic lymphocyte serpin proteinase inhibitor 9 protects against granzyme B-mediated apoptosis without perturbing the Fas cell death pathway. Mol. Cell Biol. 18: 6387. 2. Bladergroen BA, et al. The granzyme B inhibitor, protease inhibitor 9, is mainly expressed by dendritic cells and at immune privileged sites. Journal of Immunology; 2001, 166: 3218-3225. 3. Bladergroen BA, et al. Expression of the granzyme B inhibitor, protease inhibitor 9, by tumor cells in patients with non-Hodgkin and Hodgkin lymphoma: a novel protective mechanism for tumor cells to circumvent the immune system? Blood. 2002 Jan 1;99(1):232-7.

Additional Info

Recognizes human SERPINB9.