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SET 9

Cat no: S1012-24

SET 9

Specifically transfers a methyl group at lysine 4 of histone H3. Some labeling of histone H4 has been observed if no other histones are present in the reaction.\n\nSimilar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family.\n\nSpecific Activity: ~30mU/mg, where 1 unit of SET9 activity is equal to 1nmol of methyl transferred into histone H3 per minute using S-Adenosyl LMethionine (SAM) as donor.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20 degrees C or colder. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1012-24

Size

100ug

Form

Supplied as a liquid in PBS, pH 7.4, 200mM imidazole, 1mM PMSF, 0.1% Triton X-100 and 10% glycerol.

Purity

Ni-NTA agarose chromatography.

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