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SETA (CIN85, Ruk, SH3KBPI)

Cat no: S1012-10

SETA (CIN85, Ruk, SH3KBPI)

SETA (CIN85, Ruk/SH3KBPI) regulates the ligand-dependent endocytosis of the IgE receptor.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml detects SETA in RIPA lysates from NG108-15 cells.\nImmunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate SETA in human U87 glioma cells and rat primary astrocytes.\nImmunocytochemistry: Has been reported by an independent laboratory to detect SETA in rat primary astrocytes.\nOptimal dilutions to be determined by the researcher. \n\nRecommended secondary antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1012-10

Size

200ug

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 0.1M Tris glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Finniss, S., et al., Biochem. Biophys. Res. Comm. 325: 174-182, 2004. 2. Chen, B., et al., J. Biol. Chem. 275: 19275-19281, 2000. 3. Bogler, O., et al., Neuro-Oncology 2: 6-15, 2000.

Additional Info

Recognizes rat SETA, Mr ~85kD. SETA has multiple splice variants which can result in multiple bands in Western Blot. Species Crossreactivity: human, mouse.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

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