sFas Ligand, Recombinant, Human (soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL)

Fas Ligand (FasL) is a member of the TNF superfamily that is expressed on the cell surface of activated T cells.Binding of FasL to Fas Receptor triggers apoptosis in Fas-bearing cells.FasL has the ability to kill T cells and activated B cells which leads to down-regulation of the immune response.The mechanism of Fas induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms.These active caspases then cleave various cellular substrates leading to the eventual cell death.Both human and murine sFasL are fully active on human and murine cells.Recombinant human soluble Fas Ligand is a 17.9kD protein comprising the TNF homologous region of FasL and contains an 8 residue N-terminal His-Tag.\n\nBiological Activity:\nDetermined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ~10.0ng/ml, corresponding to a specific activity of ~1x10e5 units/mg.\n\nAA Sequence:\nHHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL\n\nCrossreactivity:\nMouse\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts