sFas Receptor, Recombinant, Human (soluble Fas receptor (sFasR), TNFRSF6, CD95, Apo I, Fas Antigen)

Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively.Binding of FasL to Fas triggers apoptosis in Fas-bearing cells.The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms.These active caspases then cleave various cellular substrates leading to the eventual cell death.sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.Recombinant human soluble Fas (sFas Receptor) is a 157 amino acid polypeptide (17.6kD) corresponding to the TNFR homologous cysteine rich extracellular domain Fas.\n\nBiological Activity:\nThe ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15ug/ml in the presence of 2ng/ml of hFasL.\n\nAA Sequence:\nMRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts