SFRP3, Recombinant, Human (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas. The N-terminal portion of the protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA (Hoang et al., 1996, J. Biol. Chem. 271:26131; Leyns et al., 1997, Cell 88:747; Wang et al., 1997, Cell 88:757; Mayr et al., 1997, Mech. Dev. 63:109; Rattner et al., 1997, PNAS 94:2859).\n\nSource: \nHuman SFRP3, Ala33-Asn325 (Accession #AAB51298), with a C-terminal 10-His tag expressed in a mouse myeloma cell line, NS0. \n\nMolecular Mass: \nBased on N-terminal sequencing, the mature human sFRP-3 protein starts at Ala33 and has a\ncalculated molecular mass of 35kD. As a result of glycosylation, the recombinant protein migrates as an ~38kD protein in SDS-PAGE under reducing conditions.\n\nEndotoxin Level: \n(same/less than)1.0EU/ug (LAL)\n\nActivity: \nMeasured by its ability to inhibit proliferation in MC3T3-E1 mouse osteoblastic cells. The ED50 is typically 1-4ug/ml.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

S1012-88R

Size

10ug

Form

Supplied as a lyophilized powder in PBS, pH 7.2, BSA.\nReconstitution: Reconstitute with 100ul sterile PBS with at least 0.1% human or bovine serum albumin.

Purity

(same/more than)95%, as determined by SDS-PAGE and visualized by silver stain.

References

1. Hoang et al., 1996, J. Biol. Chem. 271:26131. 2. Leyns et al., 1997, Cell 88:747. 3. Wang et al., 1997, Cell 88:757. 4. Mayr et al., 1997, Mech. Dev. 63:109. 5. Rattner et al., 1997, PNAS 94:2859.