SGK, active, truncated NT 60aa, Recombinant, Human (SGK1, Serum and Glucocorticoid-regulated Protein Kinase)

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family. SGK1 is activated in vitro by 3-phosphoinositide- dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase (1). SGK1, mRNA is \nexpressed in all tissues and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone. SGK1 promotes cell survival by phosphorylating and inactivating FKHRL1. SGK and Akt display differences with respect to the efficacy with which they phosphorylate the \nthree regulatory sites on FKHRL1. While both kinases can phosphorylate Thr-32, SGK1 displays a marked preference for Ser-315 whereas Akt favors Ser-253. These findings suggest that SGK1 and Akt may coordinately regulate the function of FKHRL1 by phosphorylating this transcription factor at distinct sites. Like PKB, SGK1 preferentially phosphorylates Ser and Thr residues that lie in Arg-Xaa-Arg-Xaa-Xaa- Ser/Thr motifs. The SGK1 gene has recently been identified as an \nimportant aldosterone-induced protein kinase that mediates trafficking of the renal epithelial Na(+) channel (ENaC) to the cell membrane. Thus, SGK1 is an appealing candidate for blood pressure regulation and possibly essential hypertension (3). \n\nSpecific Activity: ~60nmol/min/mg\n \nActivity Assay: \nKinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 degrees C using a final concentration of 50uM [32P] ATP.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-81E

Size

5ug

Form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

(same/more than) 90% as determined by SDS-PAGE analysis.

References

1. Kobayashi T, Deak M, Morrice N, Cohen P. (1999) Biochem J. 344 Pt 1:189-97. 2. Brunet A, Park J, Tran H, Hu LS, Hemmings BA, Greenberg ME. (2001) Mol Cell Biol. 21(3):952-65. 3. Busjahn A, Aydin A, Uhlmann R, Krasko C, Bahring S, Szelestei T, Feng Y, Dahm S, Sharma AM, Luft FC, Lang F. (2002) Hypertension. 40(3):256-60.