Shc, phosphorylated (Tyr239, Tyr240) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Shc is a signaling and transforming protein that binds Src homology 2 and 3 (SH2 and SH3) domains. Shc mediates the signaling from many types of receptors, such as those for growth factors, antigens, cytokines, integrins and G-protein coupled receptors, to their downstream proteins. This downstream signaling occurs via binding to Grb2, an adaptor protein for the Ras exchange factor Sos, leading to activation of the Ras/Raf/MAPK pathway. There are three isoforms of Shc (p46, p52 and p66), where p46 and p52 are the most widely expressed. Phosphorylation of Shc at tyrosines 239, 240 and 317 potentiates its function as a regulatory adaptor protein.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nA431 cells +/- EGF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S1010-90B1

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Ugi, S., et al. (2002) Phosphatidylinositol 3-kinase is required for insulin-stimulated tyrosine phosphorylation of Shc in 3T3-L1 adipocytes. J. Biol. Chem. 277(21):18592-18597. Hecker, T.P., et al. (2002) Focal adhesion kinase enhances signaling through the Shc/extracellular signal-regulated kinase pathway in anaplastic astrocytoma tumor biopsy samples. Cancer Res. 62(9):2699-2707. Boney, C.M., et al. (2001) Src family tyrosine kinases participate in insulin-like growth factor I mitogenic signaling in 3T3-L1 cells. Cell Growth Differ. 12(7):379-386. Barberis, L., et al. (2000) Distinct roles of the adaptor protein Shc and focal adhesion kinase in integrin signaling to ERK. J. Biol. Chem. 275(47):36532-36540. Sasaoka, T. and M. Kobayashi (2000) The functional significance of Shc in insulin signaling as a substrate of the insulin receptor. Endocr. J. 47(4):373-381. Sakaguchi, K., et al. (1998) Shc phosphotyrosine-binding domain dominantly interacts with epidermal growth factor receptors and mediates Ras activation in intact cells. Mol. Endocrinol. 12(4):536-543. Gotoh, N., et al. (1997) Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogenactivated protein kinase activation. Mol. Cell Biol. 17(4):1824-1831. Ward, C.W., et al. (1996) Systematic mapping of potential binding sites for Shc and Grb2 SH2 domains on insulin receptor substrate-1 and the receptors for insulin, epidermal growth factor, platelet-derived growth factor, and fibroblast growth factor. J. Biol. Chem. 271(10):5603-5609.

Additional Info

Recognizes human Shc when phosphorylated when Tyr239 at Tyr240. Mouse Shc has not been tested. Cross-reacts with insulin receptor in CHO cells over-expressing insulin receptor.