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Shc, Recombinant, Human, aa1-474, His-tag (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

Cat no: S1010-91G

Shc, Recombinant, Human, aa1-474, His-tag (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1)

SHC1 (SHC-transforming protein 1) belongs to the SH2 domain family. SHC1 has been found to be important in the regulation of apoptosis and drug resistance in mammalian cells. It is a signaling adapter that couples activated growth factor receptors to signaling pathway. SHC1 expressed in neural stem cells but absent in mature neurons. Purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to aa1-474 of human SHC1, fused to His-tag at N-terminal, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT \nAEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLRNPP KLVTPHDRMA GFDGSAWDEE EEEPPDHQYY NDFPGKEPPL GGVVDMRLRE GAAPGAARPT APNAQTPSHL GATLPVGQPV GGDPEVRKQM PPPPPCPAGR ELFDDPSYVN VQNLDKARQA VGGAGPPNPA \nINGSAPRDLF DMKPFEDALR VPPPPQSVSM AEQLRGEPWF HGKLSRREAE ALLQLNGDFL VRESTTTPGQ YVLTGLQSGQ PKHLLLVDPE GVVRTKDHRF ESVSHLISYH MDNHLPIISA GSELCLQQPV ERKL\n\nMolecular Weight: \n~53.8kD (494aa) \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S1010-91G

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 2mM DTT, 200mM sodium chloride, 0.1mM PMSF, 20% glycerol.

Purity

~90% (SDS-PAGE)

References

Debnath J., et al. (2010) Oncogene. 29(41):5556-8. Zhou M.-M., et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92:7784-7788.

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