Sheep anti Human Prekallikrein polyclonal antibody

Sheep Anti-Human Prekallikrein polyclonal antibody for IEP, ELISA. Prekallikrein (PK), previously known as Fletcher Factor, is a single chain glycoprotein produced in the liver. The plasma concentration of PK is 50 ?g/ml (550 nM), approximately 75% of which circulates in complex with high molecular weight kininogen (HK) and the remainder as free PK. Plasma PK is heterogeneous in both mass and charge due to variable degrees of glycosylation. Approximately 90% of plasma PK has an apparent molecular weight of 88 kDa as determined by SDS-PAGE and the remaining 10% has an apparent mass of 85 kDa. The catalytic site resides in the light chain. The heavy chain of PK contains four apple-domain structures similar to those found in F.XI and these are required for binding of PK to HK. PK is the zymogen form of the enzyme kallikrein, which is involved in the proteolysis of kininogens with subsequent release of bradykinin, a potent vasodilator. PK participates in the contact phase of coagulation as a substrate for surface-bound activated factor XII (F.XIIa) in the presence of the cofactor HK. As PK and factor XI (F.XI) both circulate in complex with HK, both are localized to activating surfaces through their respective binding to HK. Limited proteolysis of PK by F.XIIa generates kallikrein, a two-chain serine protease that initiates the reciprocal activation of PK and F.XI. Kallikrein activity in plasma is regulated predominantly by C1-Inhibitor and ?2 macroglobulin, with relatively minor contributions by Protein C Inhibitor, ?2 Antiplasmin, and antithrombin.

Prices direct from Creative Diagnostics

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

CABT-L460

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Conjugates

Unconj

Target

PK

Isotype

IgG

Clonality

Polyclonal

Storage Temp

Store between -10 and -20В°C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.